IJE TRANSACTIONS B: Applications Vol. 31, No. 8 (August 2018) 1159-1165    Article in Press

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S. Abbaspour and S. K. Sadrnezhaad
( Received: February 03, 2018 – Accepted in Revised Form: April 10, 2018 )

Abstract    Arrayed Ti6Al4V nanotubes (TNT) coated with hydroxyapatite (HA) were synthesized via electrochemical anodization method. Paracetamol was loaded onto TNT-HA electrode. Effects of anodization, nanotube formation and hydroxyapatite deposition on sorption and release of the drug were investigated. Saturation time of paracetamol on the anodized samples was 30% shorter than the hydroxyapatite-coated samples. Release behavior of the loaded drug was studied by (a) plunging the probe into phosphate buffered saline (PBS), (b) sampling the drug-loaded PBS at different times and (c) analyzing the solution via ultraviolet-visible (UV-vis) spectroscopy. Results showed that HA electrodes hold higher amounts of paracetamol than the anodized samples at longer times. Scanning electron microscopy (SEM), MTT assay, and nanoindentation tests were used to characterize the produced electrodes.


Keywords    Titanium nanotubes; Drug capacity; Drug release; Hydroxyapatite; Paracetamol


چکیده    نانولوله های ردیفی Ti6Al4V (TNT) پوشش داده شده با هیدروکسی آپاتیت (HA) با استفاده از روش آندایزینگ الکتروشیمیایی سنتز شدند. پاراستامول بر روی الکترود TNT-HA بارگذاری شد. تاثیر آندایزینگ، تشکیل نانولوله و نشستن هیدروکسی آپاتیت بر جذب و واجذب دارو مورد بررسی قرار گرفت. زمان اشباع پاراستامول بر روی نمونه های آندایز شده 30 درصدکمتر از نمونه های پوشش داده شده با هیدروکسی آپاتیت بود. رفتار رهایش داروی بارگیری شده از طریق (a) وارد کردن پروب به داخل محلول نمکی فسفات بافرشده PBS))، (b) نمونه برداری از محلول دارو دار PBS در زمان های مختلف، و (c) تجزیه محلول با استفاده از طیف سنجی با اشعه ماوراء بنفش (UV-vis) انجام شد. نتایج نشان داد که الکترودهای HA مقادیر بیشتری از پاراستامول را در مقایسه با نمونه های آندایز شده در زمان های طولانی نگهداری می کنند. برای مشخص کردن الکترودهای تولید شده از میکروسکوپ الکترونی روبسی (SEM)، آزمایش MTT و آزمون نانوایندنتیشن استفاده شد.


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