IJE TRANSACTIONS A: Basics Vol. 31, No. 4 (April 2018) 516-523    Article in Press

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A. Farahi, G. D. Najafpour and A. Ghoreyshi
( Received: December 05, 2017 – Accepted in Revised Form: January 04, 2018 )

Abstract    Broomcorn seed (Sorghum vulgare) was used as raw material for bioethanol production. Optimum conditions were obtained from response surface method. Broomcorn seed flour (45 g/l) was treated by alkaline treatment and dual enzymatic hydrolysis (0.7 g/l of α- amylase and 0.42 g/l of amyloglucosidase). The hydrolyzed total sugar of 25.5 g/L was used in conventional bioethanol production (8.1 g/l) using Saccharomyces cerevisiae. Enhanced bioethanol production was performed in membrane bioreactor (MBR) in integrated batch fermentation and membrane pervaporation process. Application of commercial polydimethylsiloxane/polyethyleneterephthalate/polyimide (PDMS/PET/PI) membrane in MBR resulted in ethanol concentration of 10.15 g/l in broth and 70.2 g/l in cold trap of MBR. Cell concentration in broth was increased from 7.2 in conventional fermentation to 9.05 g/l in MBR. In addition, ethanol production in MBR using fabricated membrane having ethanol separation factor of 8.7; ethanol concentration in broth and cold trap were 11.1 and 88.5 g/ l, respectively. Also the cell concentration of 10.2 g/l was obtained in MBR with fabricated membrane. In MBR, surface modified multi wall carbon nano tube (MWCNT) coated on membrane having ethanol separation factor of 10.2, resulted ethanol concentration of 11.9 and 110 g/l in broth and cold trap, respectively. Finally, for MBR using modified membrane the cell concentration of 11.01 g/l was obtained. Based on a comparison study, maximum ethanol separation and yield were obtained for modified membrane having MWCNT and the surface was modified by corona treatment.


Keywords    Bioethanol; Multi Walled Carbon Nano Tube; Polydimethylsiloxane; Polyethersulfune; Composite Membrane; Broomcorn Seed


چکیده    دانه­های جارو (سورگوم جارویی) به عنوان ماده اولیه در تولید بیواتانول استفاده شده است. در شرایط بهینه به دست آمده با استفاده از تکنیک پاسخ سطحی، مقدار g/l45 از دانه­های جاروی آرد شده مورد پیش تیمار قلیایی قرار گرفت و سپس با آنزیم­های آلفاآمیلاز با غلظت g/l 7/0 و آمیلوگلوکوزیداز با غلظت g/l 42/0 هیدرولیز آنزیمی گردید. قند ساده حاصل که دارای غلظت g/l 5/25 بود، در روش سنتی با مخمر Saccharomyces cerevisiae تخمیر شد و g/l 1/8 اتانول به دست آمد. دربیوراکتورغشایی و با استفاده از ترکیب عملیات تخمیر و تکنولوژی غشایی تولید اتانول افزایش یافت. با استفاده از غشای تجاری پلی دی متیل سیلوکسان/ پلی اتیلن ترفتالات/ پلی ایمید (PDMS/PET/PI)، اتانول با غلظت g/l 15/10 در بیوراکتور وg/l 2/70 در کلدترپ بیوراکتورغشایی تولید شد. مقدار رشد سلولی از از g/l 2/7 در تولید سنتی به g/l 05/9 رسید. با استفاده از غشای ساخته شده با فاکتور جداسازی 7/8 مقدار اتانول تولیدی در بیوراکتور غشایی به g/l 1/11 و در کلدترپ بیوراکتورغشایی به g/l 5/88 رسید. مقدار رشد سلولی در این حالت برابر g/l 2/10 بوده است. همچنین با استفاده از غشای پلی دی متیل سیلوکسان ساخته شده و اصلاح سطح شده و پوشیده شده با نانولوله­های کربنی چنددیواره با فاکتور جداسازی 2/10، به تولید اتانولی معادل g/l 9/11 و g/l 110 در بیوراکتور و کلدترپ بیوراکتورغشایی دست یافته شد. رشد سلولی در این حالت g/l 01/11 بود. بنابراین بیشترین اتانول تولید شده و جداسازی شده با استفاده از بیوراکتور غشایی و استفاده از غشای اصلاح سطح شده به وسیله کرونا و پوشیده شده با نانولوله­های کربنی چنددیواره می­باشد.


1.     Cantú-Lozano, D. and Luna-Solano, G., "Bioethanol production process rheology", Industrial Crops and Products,  Vol., No., (2016).

2.     El-Sebaii, A. and Shalaby, S., "Solar drying of agricultural products: A review", Renewable and Sustainable Energy Reviews,  Vol. 16, No. 1, (2012), 37-43.

3.     Peng, P., Shi, B. and Lan, Y., "A review of membrane materials for ethanol recovery by pervaporation", Separation Science and Technology,  Vol. 46, No. 2, (2010), 234-246.

4.     Wei, P., Cheng, L.-H., Zhang, L., Xu, X.-H., Chen, H.-l. and Gao, C.-j., "A review of membrane technology for bioethanol production", Renewable and Sustainable Energy Reviews,  Vol. 30, No., (2014), 388-400.

5.     Rezakazemi, M., Shahidi, K. and Mohammadi, T., "Synthetic pdms composite membranes for pervaporation dehydration of ethanol", Desalination and Water Treatment,  Vol. 54, No. 6, (2015), 1542-1549.

6.     Nasidi, M., Agu, R., Deeni, Y. and Walker, G., "Utilization of whole sorghum crop residues for bioethanol production", Journal of the Institute of Brewing,  Vol. 122, No. 2, (2016), 268-277.

7.     Farahi, A., Najafpour, G., Ghoreyshi, A., Mohammadi, M. and Esfahanian, M., "Enzymatic production of reducing sugars from broomcorn seed (sorghum vulgare): Process optimization and kinetic studies", World Applied Sciences Journal,  Vol. 18, No. 4, (2012), 568-574.

8.     Ariyajaroenwong, P., Laopaiboon, P., Salakkam, A., Srinophakun, P. and Laopaiboon, L., "Kinetic models for batch and continuous ethanol fermentation from sweet sorghum juice by yeast immobilized on sweet sorghum stalks", Journal of the Taiwan Institute of Chemical Engineers,  Vol. 66, No., (2016), 210-216.

9.     Castro, E., Nieves, I.U., Rondón, V., Sagues, W.J., Fernández-Sandoval, M.T., Yomano, L.P., York, S.W., Erickson, J. and Vermerris, W., "Potential for ethanol production from different sorghum cultivars", Industrial Crops and Products,  Vol. 109, No., (2017), 367-373.

10.   Jafari, Y., Karimi, K. and Amiri, H., "Efficient bioconversion of whole sweet sorghum plant to acetone, butanol, and ethanol improved by acetone delignification", Journal of Cleaner Production,  Vol. 166, No., (2017), 1428-1437.

11.   Singh, H. and Soni, S.K., "Production of starch-gel digesting amyloglucosidase by aspergillus oryzae hs-3 in solid state fermentation", Process Biochemistry,  Vol. 37, No. 5, (2001), 453-459.

12.   Brunetti, A., Zito, P.F., Giorno, L., Drioli, E. and Barbieri, G., "Membrane reactors for low temperature applications: An overview", Chemical Engineering and Processing: Process Intensification,  Vol., No., (2017).

13.   Trifunović, O. and Trägårdh, G., "The influence of support layer on mass transport of homologous series of alcohols and esters through composite pervaporation membranes", Journal of Membrane Science,  Vol. 259, No. 1, (2005), 122-134.

14.   Fu, C., Cai, D., Hu, S., Miao, Q., Wang, Y., Qin, P., Wang, Z. and Tan, T., "Ethanol fermentation integrated with pdms composite membrane: An effective process", Bioresource Technology,  Vol. 200, No., (2016), 648-657.

15.   Esfahanian, M., Ghorbanfarahi, A., Ghoreyshi, A., Najafpour, G., Younesi, H. and Ahmad, A., "Enhanced bioethanol production in batch fermentation by pervaporation using a pdms membrane bioreactor", International Journal of Engineering-Transactions B: Applications,  Vol. 25, No. 4, (2012), 249.

16.   Tan, Y.H., Abdullah, M.O., Nolasco-Hipolito, C. and Zauzi, N.S.A., "Application of rsm and taguchi methods for optimizing the transesterification of waste cooking oil catalyzed by solid ostrich and chicken-eggshell derived cao", Renewable Energy,  Vol. 114, No., (2017), 437-447.

17.   Verma, D., Thakur, P.S., Padhi, S., Khuroo, T., Talegaonkar, S. and Iqbal, Z., "Design expert assisted nanoformulation design for co-delivery of topotecan and thymoquinone: Optimization, in vitro characterization and stability assessment", Journal of Molecular Liquids,  Vol. 242, No., (2017), 382-394.

18.   de Oliveira Faber, M. and Ferreira-Leitão, V.S., "Optimization of biohydrogen yield produced by bacterial consortia using residual glycerin from biodiesel production", Bioresource Technology,  Vol. 219, No., (2016), 365-370.

19.   Esfahanian, M., Rad, A.S., Khoshhal, S., Najafpour, G. and Asghari, B., "Mathematical modeling of continuous ethanol fermentation in a membrane bioreactor by pervaporation compared to conventional system: Genetic algorithm", Bioresource Technology,  Vol. 212, No., (2016), 62-71.

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